The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. SWC designed and coordinated the research. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. 10K, and c M.W. Nat Biotechnol. ; Yevlampieva N.P. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Cookies policy. MeSH Banga AK. HHS Vulnerability Disclosure, Help The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. 4D imaging reveals mechanisms of clay-carbon protection and On single point determination of intrinsic viscosity. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Format), Citation-(EndNote Hydrodynamic The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Theory, Estimation, Experiment, and Data. Format), Citation-(BibTeX GPC Symp. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth Google Scholar. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are However, in vitro investigations are very few. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Polymers | Free Full-Text | Confinement-Induced Fractionation and (2003). For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. FITC-dextran solution was prepared at 1mM in PBS. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Drug Deliv. The hydrodynamic 57 radius of the dextranase is 7 Webof overall macromolecular geometry and hydrodynamic draining. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. 2003;10:33847. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. 113, 81-86. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Dextran Gold MH, Biron JA. Hydrodynamic The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. WebTry out PMC Labs and tell us what you think. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). ; van Nostrum, C.F. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. ; Patel, T.R. of different origins. All branched polysaccharide types could be described approximately by the hyperbranching theory. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). Part of (1937). FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Experimental and numerical study on the mixed lubrication J. Microporation was performed 2, 5, and 10 times on each sample. Treatment of acne scars by fractional bipolar radiofrequency energy. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Amersham Biosciences, AA Edition, www.amershambiosciences.com. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. By using this website, you agree to our Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Biomacromolecules 8, 2464-2475. An undergraduate physical chemistry experiment. ; Dutt, N.V.K. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Radius Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) Morris, G.A. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. hydrodynamic Formation of dextran hydrogels by crystallization. 2004;25(9):155967. In addition, we evaluated the regeneration of SC after microporation. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Journal of Chemical Education, 80(9), 1036-1038. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Pure Appl. Ed., 9(2), 85-90. Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). European Polymer Journal, 43, 1744-1753. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. 7 8 principles of dynamic light scattering statement and DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Soc., 21, 1, 78-86. ; Hogan, M.P. Nair SS. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Analytical Chemistry. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. Epub 2021 Feb 9. Macromolecules. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in @ Nilimesh See attached. National Library of Medicine Food Sci Biotechnol. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. A solvent at this temperature is called a theta solvent [53]. Masuelli, Martin Alberto (2013). The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Drug deliv. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. What is hydrodynamic radius One day before the experiment, the skin was stabilized in the skin culture medium. The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). Int J Food Sci Nutr. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. WebDextran 40 Pharmaceutical Quality. Walkenhorst, S.; Olivier, R. (2002). 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. Arond L.H. 1984;17(6):11703. Int J Pharm Investig. 2023 BioMed Central Ltd unless otherwise stated. AATEX. Advances in Carbohydrate Analysis, 1, 63-144. Principles of Size Exclusion Chromatography SEC (GFC Curley SA. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Bookshelf WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Carbohydrate Polymers 88 1440-1444. volume24, Articlenumber:22 (2020) Alternatives to animal testing: a review. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. (2008). 2019;9(3-S):9726. The Mark-Houwink a constant is close to 0.5 in poor solvents. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively.